The Basics of DNA Purification

Before conducting any PCR reaction or cloning experiment, or even DNA sequencing, it is essential to have a high-quality DNA source that is free of contaminants, such as protein, debris and RNA. The process of purifying DNA is known as DNA isolation and is one of the most important steps in molecular biology. In this article you will learn the fundamentals of DNA purification and how you can optimize your DNA extraction strategies to get more efficient results.

The initial step of the DNA purification procedure is to make a solution that consists of an amalgamation of alkaline buffer and water. This buffer makes DNA soluble, which means it is able to be separated from other components of the sample. After the DNA has been placed in an alkaline and water solution, it’s cleaned with detergents as well as salts that break down cell membranes as well as nuclei. This allows the DNA to be released. RNase may also be added to remove any contaminants in the RNA sample.

DNA is separated from other cellular components such as proteins and lipids using organic solvents like chloroform and phenol. After the DNA is separated from the proteins or lipids and lipids, it can be precipitated using alcohol or rubbing alcohol.

Gel electrophoresis and spectrophotometers can be used to determine the quality of DNA. A good quality DNA sample should have an absorbance range of 220 nm to 280 nm. 1.8. A low ratio may indicate a problem with the protein binding process or the carryover of salt from wash or bind buffers.